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Effie, Hailey, and Chris's book chapter is accepted for publication in Methods in Enzymology!

Near-infrared photoactivatable nitric oxide donors with photoacoustic readout

In this chapter, we motivate the need for photoactivatable NO donor molecules and give a brief survey of the existing chemical tools in the field. We then provide detailed protocols for the synthesis and validation of a near-infrared light-activated NO donor molecule, photoNOD-1, developed in our research group. With this tool, NO can be released in vivo in a radiation-dependent manner that can be monitored using photoacoustic imaging.

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