Anuj and Chris's paper is published in Angewandte Chemie!

December 18, 2019

An activity-based sensing approach for the detection of cyclooxygenase-2 in live cells

 

Cyclooxygenase‐2 (COX‐2) overexpression is prominent in inflammatory diseases, neurodegenerative disorders, and cancer. Directly monitoring COX‐2 activity within its native environment poses an exciting approach to account for and illuminate the effect of the local environments on protein activity. Herein, we report the development of CoxFluor, the first activity‐based sensing approach for monitoring COX‐2 within live cells with confocal microscopy and flow cytometry. CoxFluor strategically links a natural substrate with a dye precursor to engage both the cyclooxygenase and peroxidase activities of COX‐2. This catalyzes the release of resorufin and the natural product, as supported by molecular dynamics and ensemble docking. CoxFluor enabled the detection of oxygen‐dependent changes in COX‐2 activity that are independent of protein expression within live macrophage cells.

 

article

 

Please reload

Featured Posts

Chris and Irian's paper is published in Chemical Science!

January 6, 2020

1/10
Please reload

Recent Posts
Search By Tags

October 28, 2019

October 28, 2019

October 28, 2019

Please reload

Please reload

Follow Us
  • Facebook Classic
  • Twitter Classic
  • Google Classic

© #CHANLAB 2020.

  • w-facebook
  • Twitter Clean